Associate Research Professor in the Department of Chemistry
Associate Research Professor of Physics (Secondary)
Faculty Network Member of the Duke Institute for Brain Sciences
Dr. Fischer’s research focuses on exploring novel nonlinear optical contrast mechanisms for molecular imaging. Nonlinear optical microscopes can provide non-invasive, high-resolution, 3-dimensional images even in highly scattering environments such as biological tissue.
Established contrast mechanisms, such as two-photon fluorescence or harmonic generation, can image a range of targets (such as autofluorescent markers or some connective tissue structure), but many of the most molecularly specific nonlinear interactions are harder to measure with power levels one might be willing to put on tissue. In order to use these previously inaccessible interactions as structural and molecular image contrasts we are developing ultrafast laser pulse shaping and pulse shape detection methods that dramatically enhance measurement sensitivity. Applications of these microscopy methods range from imaging biological tissue (mapping structure, endogenous tissue markers, or exogenous contrast agents) to characterization of nanomaterials (such as graphene and gold nanoparticles). The molecular contrast mechanisms we originally developed for biomedical imaging also provide pigment-specific signatures for paints used in historic artwork. Recently we have demonstrated that we can noninvasively image paint layers in historic paintings and we are currently developing microscopy techniques for use in art conservation and conservation science.
Wilson, Jesse W., et al. “Optical clearing of archive-compatible paraffin embedded tissue for multiphoton microscopy: erratum.” Biomedical Optics Express, vol. 4, no. 2, The Optical Society, Feb. 2013, pp. 219–219. Crossref, doi:10.1364/boe.4.000219. Full Text
Li, Baolei, et al. “Direct Optical Imaging of Graphene In Vitro by Nonlinear Femtosecond Laser Spectral Reshaping.” Nano Letters, vol. 12, no. 11, American Chemical Society (ACS), Nov. 2012, pp. 5936–40. Crossref, doi:10.1021/nl303358p. Full Text
Wilson, Jesse W., et al. “Optical clearing of archive-compatible paraffin embedded tissue for multiphoton microscopy.” Biomedical Optics Express, vol. 3, no. 11, The Optical Society, Nov. 2012, pp. 2752–2752. Crossref, doi:10.1364/boe.3.002752. Full Text
Robles, Francisco E., et al. “Phasor analysis for nonlinear pump-probe microscopy.” Optics Express, vol. 20, no. 15, The Optical Society, July 2012, pp. 17082–17082. Crossref, doi:10.1364/oe.20.017082. Full Text
Li, Baolei, et al. “Multicontrast nonlinear optical microscopy with a compact and rapid pulse shaper.” Optics Letters, vol. 37, no. 13, The Optical Society, July 2012, pp. 2763–2763. Crossref, doi:10.1364/ol.37.002763. Full Text
Wilson, Jesse W., et al. “Cross-phase modulation spectral shifting: nonlinear phase contrast in a pump-probe microscope.” Biomedical Optics Express, vol. 3, no. 5, The Optical Society, May 2012, pp. 854–854. Crossref, doi:10.1364/boe.3.000854. Full Text
Samineni, Prathyush, et al. “Pump-probe imaging of historical pigments used in paintings.” Optics Letters, vol. 37, no. 8, The Optical Society, Apr. 2012, pp. 1310–1310. Crossref, doi:10.1364/ol.37.001310. Full Text
Zhang, R., et al. “Controllable ultrabroadband slow light in a warm rubidium vapor.” Journal of the Optical Society of America B: Optical Physics, vol. 28, no. 11, 2011, pp. 2578–83. Scival, doi:10.1364/JOSAB.28.002578. Full Text Open Access Copy
Samineni, P., et al. “Nonlinear phase contrast imaging in neuronal tissue.” Optics Infobase Conference Papers, 2011.
Li, B., et al. “Enhancing coherent anti-stokes Raman scattering background suppression with phase cycled structured femtosecond laser pulses.” Optics Infobase Conference Papers, 2010.
Samineni, P., et al. “Femtosecond laser pulse shaping improves self-phase modulation measurements in scattering media.” Optics Infobase Conference Papers, 2010.
Warren, W. S., et al. “Nonlinear microscopy without fluorescence: Seeing the needle in the Haystack with Femtosecond pulse shaping.” Optics Infobase Conference Papers, 2009.
Fischer, M. C., et al. “Enhancing two-color absorption, self-phase modulation and raman microscopy signatures in tissue with femtosecond laser pulse shaping.” Progress in Biomedical Optics and Imaging Proceedings of Spie, vol. 7183, 2009. Scopus, doi:10.1117/12.816488. Full Text
Warren, W. S. “Tissue imaging with shaped femtosecond laser pulses.” Optics Infobase Conference Papers, 2009.
Warren, W. S., et al. “New nonlinear signatures in spectroscopy and imaging.” Conference on Quantum Electronics and Laser Science (Qels) Technical Digest Series, 2008. Scopus, doi:10.1109/QELS.2008.4552901. Full Text
Piletic, I. R., et al. “Accessing nonlinear contrast in imaging using rapid pulse shaping techniques.” Optics Infobase Conference Papers, 2008.
Fischer, M. C., et al. “Femtosecond laser pulse shaping for molecular imaging in biological tissue.” Optics Infobase Conference Papers, 2008.
Liu, H. C., et al. “Intrinsic nonlinear optical signatures of neuronal activity.” Optics Infobase Conference Papers, 2008. Scopus, doi:10.1364/fio.2008.fwd2. Full Text