Associate Research Professor in the Department of Chemistry
Associate Research Professor of Physics (Secondary)
Faculty Network Member of the Duke Institute for Brain Sciences
Dr. Fischer’s research focuses on exploring novel nonlinear optical contrast mechanisms for molecular imaging. Nonlinear optical microscopes can provide non-invasive, high-resolution, 3-dimensional images even in highly scattering environments such as biological tissue.
Established contrast mechanisms, such as two-photon fluorescence or harmonic generation, can image a range of targets (such as autofluorescent markers or some connective tissue structure), but many of the most molecularly specific nonlinear interactions are harder to measure with power levels one might be willing to put on tissue. In order to use these previously inaccessible interactions as structural and molecular image contrasts we are developing ultrafast laser pulse shaping and pulse shape detection methods that dramatically enhance measurement sensitivity. Applications of these microscopy methods range from imaging biological tissue (mapping structure, endogenous tissue markers, or exogenous contrast agents) to characterization of nanomaterials (such as graphene and gold nanoparticles). The molecular contrast mechanisms we originally developed for biomedical imaging also provide pigment-specific signatures for paints used in historic artwork. Recently we have demonstrated that we can noninvasively image paint layers in historic paintings and we are currently developing microscopy techniques for use in art conservation and conservation science.
Li, B., et al. “Optimizing shape of femtosecond laser pulses for homodyne detection of nonlinear optical signals.” Optics Infobase Conference Papers, Dec. 2011.
Fischer, M. C., et al. “Accessing nonlinear phase contrast in biological tissue using femtosecond laser pulse shaping.” Optics Infobase Conference Papers, Dec. 2011.
Samineni, P., et al. “Cross-phase modulation microscopy.” Optics Infobase Conference Papers, Dec. 2011.
Fischer, M. C., et al. “Accessing nonlinear phase contrast in biological tissue using femtosecond laser pulse shaping.” Progress in Biomedical Optics and Imaging Proceedings of Spie, vol. 8086, July 2011. Scopus, doi:10.1117/12.889668. Full Text
Samineni, P., et al. “Nonlinear phase contrast imaging in neuronal tissue.” Optics Infobase Conference Papers, 2011.
Samineni, P., et al. “Nonlinear phase contrast imaging in neuronal tissue.” Optics Infobase Conference Papers, Optical Society of America, 2011.
Zhang, R., et al. “Controllable ultrabroadband slow light in a warm rubidium vapor.” Journal of the Optical Society of America B: Optical Physics, vol. 28, no. 11, 2011, pp. 2578–83. Scival, doi:10.1364/JOSAB.28.002578. Full Text Open Access Copy
Li, B., et al. “Enhancing coherent anti-stokes Raman scattering background suppression with phase cycled structured femtosecond laser pulses.” Optics Infobase Conference Papers, Dec. 2010.
Samineni, P., et al. “Femtosecond laser pulse shaping improves self-phase modulation measurements in scattering media.” Optics Infobase Conference Papers, Dec. 2010.
Li, Baolei, et al. “Phase-cycling coherent anti-Stokes Raman scattering using shaped femtosecond laser pulses..” Optics Express, vol. 18, no. 25, Dec. 2010, pp. 25825–32. Epmc, doi:10.1364/oe.18.025825. Full Text