Adam P. Wax
Professor of Biomedical Engineering
Professor of Physics (Secondary)
Faculty Network Member of the Duke Institute for Brain Sciences
Member of the Duke Cancer Institute
Dr. Wax's research interests include optical spectroscopy for early cancer detection, novel microscopy and
The study of intact, living cells with optical spectroscopy offers the opportunity to observe cellular structure, organization and dynamics in a way that is not possible with traditional methods. We have developed a set of novel spectroscopic techniques for measuring spatial, temporal and refractive structure on sub-hertz and sub-wavelength scales based on using low-coherence interferometry (LCI) to detect scattered light. We have applied these techniques in different types of cell biology experiments. In one experiment, LCI measurements of the angular pattern of backscattered light are used to determine non-invasively the structure of sub-cellular organelles in cell monolayers, and the components of epithelial tissue from freshly excised rat esophagus. This work has potential as a diagnostic method for early cancer detection. In another experiment, LCI phase measurements are used to examine volume changes of epithelial cells in a monolayer in response to environmental osmolarity changes. Although cell volume changes have been measured previously, this work demonstrates for the first time the volume of just a few cells (2 or 3) tracked continuously and in situ.
Robles, F. E., and A. Wax. “Spectroscopic optical coherence tomography for quantitative molecular imaging.” Optics Infobase Conference Papers, Jan. 2011. Scopus, doi:10.1364/omp.2011.otub4. Full Text
Shaked, N. T., and A. Wax. “Quantitative analysis of three-dimensional biological cells using interferometric microscopy.” Proceedings of Spie the International Society for Optical Engineering, vol. 8043, Jan. 2011. Scopus, doi:10.1117/12.882357. Full Text
Drake, T., et al. “Multimodal optical detection of intravaginal microbicide gel coating thickness distribution.” Optics Infobase Conference Papers, 2011.
Crow, Matthew J., et al. “Plasmonic flow cytometry by immunolabeled nanorods.” Cytometry A, vol. 79, no. 1, Jan. 2011, pp. 57–65. Pubmed, doi:10.1002/cyto.a.20994. Full Text
Robles, F. E., and A. Wax. “Spectroscopic optical coherence tomography for quantitative molecular imaging.” Optics Infobase Conference Papers, 2011.
Wax, Adam, and Kevin J. Chalut. “Nuclear morphology measurements with angle-resolved low coherence interferometry for application to cell biology and early cancer detection.” Analytical Cellular Pathology (Amsterdam), vol. 34, no. 5, Jan. 2011, pp. 207–22. Epmc, doi:10.3233/acp-2011-0017. Full Text
Zhu, Yizheng, et al. “Design and validation of an angle-resolved low-coherence interferometry fiber probe for in vivo clinical measurements of depth-resolved nuclear morphology.” Journal of Biomedical Optics, vol. 16, no. 1, Jan. 2011, p. 011003. Epmc, doi:10.1117/1.3520130. Full Text
Terry, Neil G., et al. “Detection of dysplasia in Barrett's esophagus with in vivo depth-resolved nuclear morphology measurements.” Gastroenterology, vol. 140, no. 1, Jan. 2011, pp. 42–50. Epmc, doi:10.1053/j.gastro.2010.09.008. Full Text
Wax, A. “Coherence imaging.” Optics Infobase Conference Papers, Dec. 2010.
Chowdhury, S., et al. “Detecting hemoglobin concentration using the dual window method for processing spectroscopic optical coherence tomography signals.” Optics Infobase Conference Papers, Dec. 2010.