Adam P. Wax
Professor of Biomedical Engineering
Professor of Physics (Secondary)
Faculty Network Member of the Duke Institute for Brain Sciences
Member of the Duke Cancer Institute
Dr. Wax's research interests include optical spectroscopy for early cancer detection, novel microscopy and
The study of intact, living cells with optical spectroscopy offers the opportunity to observe cellular structure, organization and dynamics in a way that is not possible with traditional methods. We have developed a set of novel spectroscopic techniques for measuring spatial, temporal and refractive structure on sub-hertz and sub-wavelength scales based on using low-coherence interferometry (LCI) to detect scattered light. We have applied these techniques in different types of cell biology experiments. In one experiment, LCI measurements of the angular pattern of backscattered light are used to determine non-invasively the structure of sub-cellular organelles in cell monolayers, and the components of epithelial tissue from freshly excised rat esophagus. This work has potential as a diagnostic method for early cancer detection. In another experiment, LCI phase measurements are used to examine volume changes of epithelial cells in a monolayer in response to environmental osmolarity changes. Although cell volume changes have been measured previously, this work demonstrates for the first time the volume of just a few cells (2 or 3) tracked continuously and in situ.
Shaked, N. T., and A. Wax. “New directions in interferometric phase microscopy of biological cell dynamics.” Optics Infobase Conference Papers, Dec. 2010.
Robles, Francisco E., and Adam Wax. “Separating the scattering and absorption coefficients using the real and imaginary parts of the refractive index with low-coherence interferometry.” Optics Letters, vol. 35, no. 17, Sept. 2010, pp. 2843–45. Epmc, doi:10.1364/ol.35.002843. Full Text Open Access Copy
Robles, Francisco E., et al. “Detection of early colorectal cancer development in the azoxymethane rat carcinogenesis model with Fourier domain low coherence interferometry.” Biomedical Optics Express, vol. 1, no. 2, Aug. 2010, pp. 736–45. Epmc, doi:10.1364/boe.1.000736. Full Text
Shaked, Natan T., et al. “Whole-cell-analysis of live cardiomyocytes using wide-field interferometric phase microscopy.” Biomedical Optics Express, vol. 1, no. 2, Aug. 2010, pp. 706–19. Epmc, doi:10.1364/boe.1.000706. Full Text Open Access Copy
Rinehart, Matthew T., et al. “Simultaneous two-wavelength transmission quantitative phase microscopy with a color camera.” Optics Letters, vol. 35, no. 15, Aug. 2010, pp. 2612–14. Epmc, doi:10.1364/ol.35.002612. Full Text Open Access Copy
Robles, Francisco E., et al. “Assessing hemoglobin concentration using spectroscopic optical coherence tomography for feasibility of tissue diagnostics.” Biomedical Optics Express, vol. 1, no. 1, July 2010, pp. 310–17. Epmc, doi:10.1364/boe.1.000310/. Full Text
Giacomelli, M., et al. “Size and shape determination of spheroidal scatterers using two-dimensional angle resolved scattering.” Optics Express, vol. 18, no. 14, July 2010, pp. 14616–26.
Giacomelli, M. G., et al. “Review of the application of T-matrix calculations for determining the structure of cell nuclei with angle-resolved light scattering measurements.” Ieee Journal on Selected Topics in Quantum Electronics, vol. 16, no. 4, July 2010, pp. 900–08. Scopus, doi:10.1109/JSTQE.2009.2031984. Full Text
Giacomelli, Michael, et al. “Size and shape determination of spheroidal scatterers using two-dimensional angle resolved scattering.” Optics Express, vol. 18, no. 14, July 2010, pp. 14616–26. Epmc, doi:10.1364/oe.18.014616. Full Text Open Access Copy
Ehlers, M. D., et al. Parallel on-axis holographic phase microscopy of biological cells and unicellular microorganism dynamics. May 2010. Open Access Copy