Adam P. Wax

Adam P. Wax

Professor of Biomedical Engineering

Professor of Physics (Secondary)

Faculty Network Member of the Duke Institute for Brain Sciences

Member of the Duke Cancer Institute

Bass Fellow

Office Location: 
2571 CIEMAS, Durham, NC 27708
Front Office Address: 
Box 90281, Durham, NC 27708-0281
Phone: 
(919) 660-5143

Overview

Dr. Wax's research interests include optical spectroscopy for early cancer detection, novel microscopy and
interferometry techniques.

The study of intact, living cells with optical spectroscopy offers the opportunity to observe cellular structure, organization and dynamics in a way that is not possible with traditional methods. We have developed a set of novel spectroscopic techniques for measuring spatial, temporal and refractive structure on sub-hertz and sub-wavelength scales based on using low-coherence interferometry (LCI) to detect scattered light. We have applied these techniques in different types of cell biology experiments. In one experiment, LCI measurements of the angular pattern of backscattered light are used to determine non-invasively the structure of sub-cellular organelles in cell monolayers, and the components of epithelial tissue from freshly excised rat esophagus. This work has potential as a diagnostic method for early cancer detection. In another experiment, LCI phase measurements are used to examine volume changes of epithelial cells in a monolayer in response to environmental osmolarity changes. Although cell volume changes have been measured previously, this work demonstrates for the first time the volume of just a few cells (2 or 3) tracked continuously and in situ.

Education & Training

  • Ph.D., Duke University 1999

  • M.A., Duke University 1996

  • B.S., Rensselaer Polytechnic Institute 1993

Rinehart, Matthew T., et al. “Simultaneous two-wavelength transmission quantitative phase microscopy with a color camera.Optics Letters, vol. 35, no. 15, Aug. 2010, pp. 2612–14. Epmc, doi:10.1364/ol.35.002612. Full Text Open Access Copy

Robles, Francisco E., et al. “Assessing hemoglobin concentration using spectroscopic optical coherence tomography for feasibility of tissue diagnostics.Biomedical Optics Express, vol. 1, no. 1, July 2010, pp. 310–17. Epmc, doi:10.1364/boe.1.000310/. Full Text

Giacomelli, M., et al. “Size and shape determination of spheroidal scatterers using two-dimensional angle resolved scattering.Optics Express, vol. 18, no. 14, July 2010, pp. 14616–26.

Giacomelli, M. G., et al. “Review of the application of T-matrix calculations for determining the structure of cell nuclei with angle-resolved light scattering measurements.” Ieee Journal on Selected Topics in Quantum Electronics, vol. 16, no. 4, July 2010, pp. 900–08. Scopus, doi:10.1109/JSTQE.2009.2031984. Full Text

Giacomelli, Michael, et al. “Size and shape determination of spheroidal scatterers using two-dimensional angle resolved scattering.Optics Express, vol. 18, no. 14, July 2010, pp. 14616–26. Epmc, doi:10.1364/oe.18.014616. Full Text Open Access Copy

Robles, F. E., and A. Wax. “Measuring structural features using a dual window method for light scattering spectroscopy and Fourier-domain low coherence interferometry.” Progress in Biomedical Optics and Imaging  Proceedings of Spie, vol. 7573, May 2010. Scopus, doi:10.1117/12.842375. Full Text

Terry, N. G., et al. “Fourier-domain angle-resolved low coherence interferometry for clinical detection of dysplasia.” Progress in Biomedical Optics and Imaging  Proceedings of Spie, vol. 7573, May 2010. Scopus, doi:10.1117/12.842464. Full Text

Shaked, Natan T., et al. “Reflective interferometric chamber for quantitative phase imaging of biological sample dynamics.Journal of Biomedical Optics, vol. 15, no. 3, May 2010, p. 030503. Epmc, doi:10.1117/1.3420179. Full Text

Shaked, Natan T., et al. “Parallel on-axis holographic phase microscopy of biological cells and unicellular microorganism dynamics.Applied Optics, vol. 49, no. 15, May 2010, pp. 2872–78. Epmc, doi:10.1364/ao.49.002872. Full Text Open Access Copy

Zhu, Yizheng, et al. “Fiber-optic interferometric two-dimensional scattering-measurement system.Optics Letters, vol. 35, no. 10, May 2010, pp. 1641–43. Epmc, doi:10.1364/ol.35.001641. Full Text Open Access Copy

Pages