Adam P. Wax

Adam P. Wax

Professor of Biomedical Engineering

Professor of Physics (Secondary)

Faculty Network Member of the Duke Institute for Brain Sciences

Member of the Duke Cancer Institute

Bass Fellow

Office Location: 
2571 CIEMAS, Durham, NC 27708
Front Office Address: 
Box 90281, Durham, NC 27708-0281
Phone: 
(919) 660-5143

Overview

Dr. Wax's research interests include optical spectroscopy for early cancer detection, novel microscopy and
interferometry techniques.

The study of intact, living cells with optical spectroscopy offers the opportunity to observe cellular structure, organization and dynamics in a way that is not possible with traditional methods. We have developed a set of novel spectroscopic techniques for measuring spatial, temporal and refractive structure on sub-hertz and sub-wavelength scales based on using low-coherence interferometry (LCI) to detect scattered light. We have applied these techniques in different types of cell biology experiments. In one experiment, LCI measurements of the angular pattern of backscattered light are used to determine non-invasively the structure of sub-cellular organelles in cell monolayers, and the components of epithelial tissue from freshly excised rat esophagus. This work has potential as a diagnostic method for early cancer detection. In another experiment, LCI phase measurements are used to examine volume changes of epithelial cells in a monolayer in response to environmental osmolarity changes. Although cell volume changes have been measured previously, this work demonstrates for the first time the volume of just a few cells (2 or 3) tracked continuously and in situ.

Education & Training

  • Ph.D., Duke University 1999

  • M.A., Duke University 1996

  • B.S., Rensselaer Polytechnic Institute 1993

Selected Grants

Duke CTSA (TL1) awarded by National Institutes of Health (Mentor). 2018 to 2023

Angle resolved light scattering through a multimode fiber for measuring cell nuclei morphology awarded by National Science Foundation (Principal Investigator). 2020 to 2023

Analysis of mechanical induction of bioelectric activity in cells awarded by Army Research Office (Principal Investigator). 2019 to 2022

Advanced a/LCI systems for improved clinical utility awarded by National Institutes of Health (Principal Investigator). 2016 to 2021

Novel Coherence Imaging to Evaluate the Health of the Cervical Epithelium awarded by National Institutes of Health (Principal Investigator). 2014 to 2021

High throughput cell screening for toxic metal exposure awarded by National Institutes of Health (Principal Investigator). 2018 to 2020

Deep skin imaging with OCT awarded by National Science Foundation (Principal Investigator). 2018 to 2020

Training in Medical Imaging awarded by National Institutes of Health (Mentor). 2003 to 2020

Coherent light scattering for early detection of Alzheimer's disease awarded by National Institutes of Health (Principal Investigator). 2017 to 2020

Visualization of mechanical stress in live cells awarded by National Science Foundation (Principal Investigator). 2016 to 2019

Pages

Graf, R. N., et al. “Assessing microscopic structural features using fourier-domain low coherence interferometry.” Handbook of Biomedical Optics, 2016, pp. 463–82.

Wax, A., et al. “Elastic scattering spectroscopy and optical coherence tomography.” Optical Coherence Tomography: Technology and Applications, Second Edition, 2015, pp. 1207–35. Scopus, doi:10.1007/978-3-319-06419-2_38. Full Text

Zhu, Y., et al. “Interferometric light scattering techniques for measuring nuclear morphology and detecting dysplasia.” Biomedical Photonics Handbook, 2014, pp. 653–81.

Vo-Dinh, T. Preface. 2014, pp. ix–xi. Scopus, doi:10.1201/b17289. Full Text

Wax, A., and F. E. Robles. “Molecular imaging true color spectroscopic (METRiCS) optical coherence tomography.” Medical Imaging: Technology and Applications, 2013, pp. 53–87. Scopus, doi:10.1201/b15511. Full Text

Zhang, Haoran, et al. “Reconstruction of angle-resolved backscattering through a multimode fiber for cell nuclei and particle size determination.” Apl Photonics, vol. 5, no. 7, AIP Publishing, July 2020, pp. 076105–076105. Crossref, doi:10.1063/5.0011500. Full Text

Song, Ge, et al. “Multimodal Coherent Imaging of Retinal Biomarkers of Alzheimer's Disease in a Mouse Model.Sci Rep, vol. 10, no. 1, May 2020, p. 7912. Pubmed, doi:10.1038/s41598-020-64827-2. Full Text

Corrigendum: Origin of improved depth penetration in dual‐axis optical coherence tomography: a Monte Carlo study.” Journal of Biophotonics, vol. 13, no. 1, Wiley, Jan. 2020. Crossref, doi:10.1002/jbio.201990013. Full Text

Jelly, Evan T., et al. “Optical coherence tomography through a rigid borescope applied to quantification of articular cartilage thickness in a porcine knee model.Optics Letters, vol. 44, no. 22, Nov. 2019, pp. 5590–93. Epmc, doi:10.1364/ol.44.005590. Full Text

Muñoz, Alexandra, et al. “Corrigendum: Cellular shear stiffness reflects progression of arsenic-induced transformation during G1.Carcinogenesis, vol. 40, no. 10, Oct. 2019, p. 1298. Epmc, doi:10.1093/carcin/bgz048. Full Text

Eldridge, Will J., et al. “Shear Modulus Measurement by Quantitative Phase Imaging and Correlation with Atomic Force Microscopy.Biophysical Journal, vol. 117, no. 4, Aug. 2019, pp. 696–705. Epmc, doi:10.1016/j.bpj.2019.07.008. Full Text

Zhao, Yang, et al. “Origin of improved depth penetration in dual-axis optical coherence tomography: a Monte Carlo study.Journal of Biophotonics, vol. 12, no. 6, June 2019, p. e201800383. Epmc, doi:10.1002/jbio.201800383. Full Text

Song, Ge, et al. “First Clinical Application of Low-Cost OCT.Translational Vision Science & Technology, vol. 8, no. 3, May 2019, p. 61. Epmc, doi:10.1167/tvst.8.3.61. Full Text

Steelman, Z. A., et al. “Light-scattering methods for tissue diagnosis.” Optica, vol. 6, no. 4, Apr. 2019, pp. 479–89. Scopus, doi:10.1364/OPTICA.6.000479. Full Text

Zhang, Haoran, et al. “Angular range, sampling and noise considerations for inverse light scattering analysis of nuclear morphology.Journal of Biophotonics, vol. 12, no. 2, Feb. 2019, p. e201800258. Epmc, doi:10.1002/jbio.201800258. Full Text

Pages

Jelly, E. T., et al. “Expanded imaging volume for dual-axis optical coherence tomography.” Frontiers in Optics  Proceedings Frontiers in Optics + Laser Science Aps/Dls, 2019. Scopus, doi:10.1364/FIO.2019.FM3C.3. Full Text

Jelly, E. T., and A. Wax. “Arthroscopic delivery of OCT using low-cost OCT system for assessment of porcine articular cartilage thickness.” Bio Optics: Design and Application  Proceedings Biophotonics Congress: Optics in the Life Sciences Congress 2019 (Boda, Brain, Ntm, Oma, Omp), 2019. Scopus, doi:10.1364/BODA.2019.DT2B.4. Full Text

Zhang, H., et al. “Computational reconstruction of angular scattering distributions through an individual multimode fiber.” Bio Optics: Design and Application  Proceedings Biophotonics Congress: Optics in the Life Sciences Congress 2019 (Boda, Brain, Ntm, Oma, Omp), 2019. Scopus, doi:10.1364/BODA.2019.JW4C.6. Full Text

Zhao, Y., et al. “Enhanced depth penetration by dual-axis optical coherence tomography.” Progress in Biomedical Optics and Imaging  Proceedings of Spie, vol. 10867, 2019. Scopus, doi:10.1117/12.2507242. Full Text

Jelly, E. T., et al. “Expanded imaging volume for dual-axis optical coherence tomography.” Optics Infobase Conference Papers, vol. Part F144-FiO 2019, 2019. Scopus, doi:10.1364/FIO.2019.FMC.3. Full Text

Song, G., et al. “Design and implementation of a low-cost, portable OCT system.” Optics Infobase Conference Papers, vol. Part F90-OTS 2018, 2018. Scopus, doi:10.1364/OTS.2018.OF3D.7. Full Text

Zhao, Y., et al. “Speckle reduction in optical coherence tomography at video rate.” Optics Infobase Conference Papers, vol. Part F90-OTS 2018, 2018. Scopus, doi:10.1364/OTS.2018.OF3D.3. Full Text

Jelly, E. T., et al. “Adaptability and performance of low-cost OCT System for use in Benchtop optical coherence microscopy.” Optics Infobase Conference Papers, vol. Part F91-TRANSLATIONAL 2018, 2018. Scopus, doi:10.1364/TRANSLATIONAL.2018.JW3A.32. Full Text

Ho, D., et al. “Scanning angle-resolved low coherence interferometry system for clinical detection of cervical dysplasia.” Optics Infobase Conference Papers, vol. Part F91-TRANSLATIONAL 2018, 2018. Scopus, doi:10.1364/TRANSLATIONAL.2018.CF4B.6. Full Text

Wax, A. “Development of a low-cost, portable optical coherence tomography (OCT) system.” Optics Infobase Conference Papers, vol. Part F96-AIO 2018, 2018. Scopus, doi:10.1364/AIO.2018.AW2A.4. Full Text

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