Adam P. Wax
Professor of Biomedical Engineering
Professor of Physics (Secondary)
Faculty Network Member of the Duke Institute for Brain Sciences
Member of the Duke Cancer Institute
Dr. Wax's research interests include optical spectroscopy for early cancer detection, novel microscopy and
The study of intact, living cells with optical spectroscopy offers the opportunity to observe cellular structure, organization and dynamics in a way that is not possible with traditional methods. We have developed a set of novel spectroscopic techniques for measuring spatial, temporal and refractive structure on sub-hertz and sub-wavelength scales based on using low-coherence interferometry (LCI) to detect scattered light. We have applied these techniques in different types of cell biology experiments. In one experiment, LCI measurements of the angular pattern of backscattered light are used to determine non-invasively the structure of sub-cellular organelles in cell monolayers, and the components of epithelial tissue from freshly excised rat esophagus. This work has potential as a diagnostic method for early cancer detection. In another experiment, LCI phase measurements are used to examine volume changes of epithelial cells in a monolayer in response to environmental osmolarity changes. Although cell volume changes have been measured previously, this work demonstrates for the first time the volume of just a few cells (2 or 3) tracked continuously and in situ.
Kim, S., et al. “Spectrally multiplexed photothermal OCT and novel detection methods.” Cleo: Applications and Technology, Cleo at 2012, Dec. 2012.
Drake, Tyler K., et al. “In vivo optical imaging of human vaginal gel thickness distributions with a probe-based, dual-modality instrument.” Journal of Biomedical Optics, vol. 17, no. 11, Nov. 2012, p. 116014. Epmc, doi:10.1117/1.jbo.17.11.116014. Full Text
Kim, Sanghoon, et al. “Phase-sensitive OCT imaging of multiple nanoparticle species using spectrally multiplexed single pulse photothermal excitation.” Biomedical Optics Express, vol. 3, no. 10, Oct. 2012, pp. 2579–86. Epmc, doi:10.1364/boe.3.002579. Full Text
Li, You Leo, et al. “Multispectral nanoparticle contrast agents for true-color spectroscopic optical coherence tomography.” Biomedical Optics Express, vol. 3, no. 8, Aug. 2012, pp. 1914–23. Epmc, doi:10.1364/boe.3.001914. Full Text
Giacomelli, M. G., and A. Wax. “Imaging contrast and resolution in multiply scattered low coherence interferometry.” Ieee Journal on Selected Topics in Quantum Electronics, vol. 18, no. 3, June 2012, pp. 1050–58. Scopus, doi:10.1109/JSTQE.2011.2161272. Full Text
Seekell, Kevin, et al. “Optimization of immunolabeled plasmonic nanoparticles for cell surface receptor analysis.” Methods (San Diego, Calif.), vol. 56, no. 2, Feb. 2012, pp. 310–16. Epmc, doi:10.1016/j.ymeth.2011.08.017. Full Text
Zhu, Yizheng, et al. “Angle-resolved low-coherence interferometry: an optical biopsy technique for clinical detection of dysplasia in Barrett's esophagus.” Expert Review of Gastroenterology & Hepatology, vol. 6, no. 1, Feb. 2012, pp. 37–41. Epmc, doi:10.1586/egh.11.83. Full Text
Matthews, Thomas E., and Adam Wax. “High-efficiency diffuse Raman spectroscopy through a fiber bundle.” Conference Proceedings : ... Annual International Conference of the Ieee Engineering in Medicine and Biology Society. Ieee Engineering in Medicine and Biology Society. Annual Conference, vol. 2012, Jan. 2012, pp. 1181–83. Epmc, doi:10.1109/embc.2012.6346147. Full Text