Adam P. Wax
Professor of Biomedical Engineering
Professor of Physics (Secondary)
Faculty Network Member of the Duke Institute for Brain Sciences
Member of the Duke Cancer Institute
Dr. Wax's research interests include optical spectroscopy for early cancer detection, novel microscopy and
The study of intact, living cells with optical spectroscopy offers the opportunity to observe cellular structure, organization and dynamics in a way that is not possible with traditional methods. We have developed a set of novel spectroscopic techniques for measuring spatial, temporal and refractive structure on sub-hertz and sub-wavelength scales based on using low-coherence interferometry (LCI) to detect scattered light. We have applied these techniques in different types of cell biology experiments. In one experiment, LCI measurements of the angular pattern of backscattered light are used to determine non-invasively the structure of sub-cellular organelles in cell monolayers, and the components of epithelial tissue from freshly excised rat esophagus. This work has potential as a diagnostic method for early cancer detection. In another experiment, LCI phase measurements are used to examine volume changes of epithelial cells in a monolayer in response to environmental osmolarity changes. Although cell volume changes have been measured previously, this work demonstrates for the first time the volume of just a few cells (2 or 3) tracked continuously and in situ.
Matthews, T. E., et al. “Deep tissue imaging using spectroscopic analysis of multiply scattered light.” Optica, vol. 1, no. 2, Jan. 2014, pp. 105–11. Scopus, doi:10.1364/OPTICA.1.000105. Full Text
Sun, Z., et al. “Preface.” Lecture Notes in Computer Science (Including Subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics), vol. 8833, Jan. 2014. Scopus, doi:10.1007/978-3-319-12484-1. Full Text
Rinehart, Matthew, et al. “Analysis of vaginal microbicide film hydration kinetics by quantitative imaging refractometry.” Plos One, vol. 9, no. 4, Jan. 2014, p. e95005. Epmc, doi:10.1371/journal.pone.0095005. Full Text
Maher, Jason R., et al. “Sensitivity of coded aperture Raman spectroscopy to analytes beneath turbid biological tissue and tissue-simulating phantoms.” Journal of Biomedical Optics, vol. 19, no. 11, Jan. 2014, p. 117001. Epmc, doi:10.1117/1.jbo.19.11.117001. Full Text
Matthews, Thomas E., et al. “Fourier domain multispectral multiple scattering low coherence interferometry.” Applied Optics, vol. 52, no. 34, Dec. 2013, pp. 8220–28. Epmc, doi:10.1364/ao.52.008220. Full Text
Yarmoska, S. K., et al. “A calibration standard for two-dimensional angle-resolved low-coherence interferometry.” Optical Trapping Applications, Ota 2013, Nov. 2013.
Yarmoska, S. K., et al. “A calibration standard for two-dimensional angle-resolved low-coherence interferometry.” Bio Optics: Design and Application, Boda 2013, Nov. 2013.
Zhu, Y., et al. Polarization and Spectral Interferometric Techniques for Quantitative Phase Microscopy. Aug. 2013, pp. 218–310. Scopus, doi:10.1016/B978-0-12-415871-9.00014-4. Full Text
Yarmoska, Steven K., et al. “A scattering phantom for observing long range order with two-dimensional angle-resolved Low-Coherence Interferometry.” Biomedical Optics Express, vol. 4, no. 9, Jan. 2013, pp. 1742–48. Epmc, doi:10.1364/boe.4.001742. Full Text
Wax, Adam, and Kevin J. Chalut. “Nuclear morphology measurements with angle-resolved low coherence interferometry for application to cell biology and early cancer detection.” Studies in Health Technology and Informatics, vol. 185, Jan. 2013, pp. 129–51.