Adam P. Wax

Adam P. Wax

Professor of Biomedical Engineering

Professor of Physics (Secondary)

Faculty Network Member of the Duke Institute for Brain Sciences

Member of the Duke Cancer Institute

Bass Fellow

Office Location: 
2571 CIEMAS, Durham, NC 27708
Front Office Address: 
Box 90281, Durham, NC 27708-0281
Phone: 
(919) 660-5143

Overview

Dr. Wax's research interests include optical spectroscopy for early cancer detection, novel microscopy and
interferometry techniques.

The study of intact, living cells with optical spectroscopy offers the opportunity to observe cellular structure, organization and dynamics in a way that is not possible with traditional methods. We have developed a set of novel spectroscopic techniques for measuring spatial, temporal and refractive structure on sub-hertz and sub-wavelength scales based on using low-coherence interferometry (LCI) to detect scattered light. We have applied these techniques in different types of cell biology experiments. In one experiment, LCI measurements of the angular pattern of backscattered light are used to determine non-invasively the structure of sub-cellular organelles in cell monolayers, and the components of epithelial tissue from freshly excised rat esophagus. This work has potential as a diagnostic method for early cancer detection. In another experiment, LCI phase measurements are used to examine volume changes of epithelial cells in a monolayer in response to environmental osmolarity changes. Although cell volume changes have been measured previously, this work demonstrates for the first time the volume of just a few cells (2 or 3) tracked continuously and in situ.

Education & Training

  • Ph.D., Duke University 1999

  • M.A., Duke University 1996

  • B.S., Rensselaer Polytechnic Institute 1993

Selected Grants

Coherence Imaging for Assessing Colorectal Neoplasia awarded by National Institutes of Health (Principal Investigator). 2009 to 2012

Molecular Imaging Using Hyperspectral Darkfield Microscope of Nanoparticles awarded by National Science Foundation (Principal Investigator). 2007 to 2011

Assessing Deployment of Microbicidal Gels With Label-Free Optical Measurement awarded by National Institutes of Health (Principal Investigator). 2007 to 2010

In Vivo Detection of Pre-Cancerous Lesions Using a /LCI awarded by National Institutes of Health (Principal Investigator). 2004 to 2010

Low Coherence Light Scattering for Biophotonics awarded by National Science Foundation (Principal Investigator). 2005 to 2010

CAREER: Low Coherence Light Scattering for Biophotonics awarded by National Science Foundation (Principal Investigator). 2004 to 2009

Assessing Nuclear Morphology in Thick Tissues Using fLCI awarded by National Institutes of Health (Principal Investigator). 2006 to 2008

In Vivo Detection of Pre-Cancerous Lesions Using a/LCI awarded by National Institutes of Health (Principal Investigator). 2004 to 2006

Pages

Muñoz, Alexandra, et al. “Cellular shear stiffness reflects progression of arsenic-induced transformation during G1.Carcinogenesis, vol. 39, no. 2, Feb. 2018, pp. 109–17. Epmc, doi:10.1093/carcin/bgx116. Full Text

Steelman, Zachary A., et al. “Is the nuclear refractive index lower than cytoplasm? Validation of phase measurements and implications for light scattering technologies.Journal of Biophotonics, vol. 10, no. 12, Dec. 2017, pp. 1714–22. Epmc, doi:10.1002/jbio.201600314. Full Text

Chowdhury, Shwetadwip, et al. “Structured illumination microscopy for dual-modality 3D sub-diffraction resolution fluorescence and refractive-index reconstruction.Biomedical Optics Express, vol. 8, no. 12, Dec. 2017, pp. 5776–93. Epmc, doi:10.1364/boe.8.005776. Full Text

Steelman, Zachary A., et al. “Scanning system for angle-resolved low-coherence interferometry.Optics Letters, vol. 42, no. 22, Nov. 2017, pp. 4581–84. Epmc, doi:10.1364/ol.42.004581. Full Text

Minzioni, P., et al. “Roadmap for optofluidics.” Journal of Optics (United Kingdom), vol. 19, no. 9, Aug. 2017. Scopus, doi:10.1088/2040-8986/aa783b. Full Text

Zhao, Yang, et al. “Dual-axis optical coherence tomography for deep tissue imaging.Opt Lett, vol. 42, no. 12, June 2017, pp. 2302–05. Pubmed, doi:10.1364/OL.42.002302. Full Text

Chowdhury, S., et al. “Refractive index tomography with structured illumination.” Optica, vol. 4, no. 5, May 2017, pp. 537–45. Scopus, doi:10.1364/OPTICA.4.000537. Full Text

Chowdhury, Shwetadwip, et al. “Structured illumination multimodal 3D-resolved quantitative phase and fluorescence sub-diffraction microscopy.Biomedical Optics Express, vol. 8, no. 5, May 2017, pp. 2496–518. Epmc, doi:10.1364/boe.8.002496. Full Text

Ho, Derek, et al. “Feasibility of clinical detection of cervical dysplasia using angle-resolved low coherence interferometry measurements of depth-resolved nuclear morphology.International Journal of Cancer, vol. 140, no. 6, Mar. 2017, pp. 1447–56. Epmc, doi:10.1002/ijc.30539. Full Text

Chuchuen, Oranat, et al. “Label-Free Measurements of Tenofovir Diffusion Coefficients in a Microbicide Gel Using Raman Spectroscopy.Journal of Pharmaceutical Sciences, vol. 106, no. 2, Feb. 2017, pp. 639–44. Epmc, doi:10.1016/j.xphs.2016.09.030. Full Text

Pages

Steelman, Z. A., et al. “Corruption of refractive index measurements of spheres using quantitative phase microscopy.” Optics Infobase Conference Papers, 2014. Scopus, doi:10.1364/FIO.2016.JTh2A.126. Full Text

Zhao, Y., et al. “Toward the assessment of blood oxygenation using multispectral multiple scattering low coherence interferometry.” Optics Infobase Conference Papers, 2014. Scopus, doi:10.1364/CANCER.2016.JM3A.24. Full Text

Eldridge, W. J., et al. “Linking cellular disorder strength and shear stiffness using quantitative phase imaging.” Optics Infobase Conference Papers, 2014. Scopus, doi:10.1364/FIO.2016.FF3A.6. Full Text

Kim, S., et al. “Guidance of angle-resolved low coherence interferometry using co-located optical coherence tomography on rat esophageal tissue.” Optics Infobase Conference Papers, 2014. Scopus, doi:10.1364/CANCER.2016.JTu3A.16. Full Text

Ho, D., et al. “Clinical detection of cervical dysplasia using angle-resolved low coherence interferometry.” Optics Infobase Conference Papers, 2014. Scopus, doi:10.1364/CANCER.2016.CTh4A.2. Full Text

Wax, A. “Wigner distribution: Friend to biophotonics.” Optics Infobase Conference Papers, 2013. Scopus, doi:10.1364/fio.2013.fw2d.1. Full Text

Shaked, N. T., et al. “Dynamic quantitative microscopy and nanoscopy of red blood cells in sickle cell disease.” Progress in Biomedical Optics and Imaging  Proceedings of Spie, vol. 8227, 2012. Scopus, doi:10.1117/12.907659. Full Text

Zhu, Y., et al. “Spectral-domain differential interference contrast microscopy.” Optics Infobase Conference Papers, 2011.

Zhu, Y., et al. “Spectral-domain differential interference contrast microscopy.” Optics Infobase Conference Papers, 2011. Scopus, doi:10.1364/ntm.2011.ntub4. Full Text

Rinehart, M. T., et al. “Real-time quantitative phase and dual-channel fluorescence microscopy for studying cellular and biomolecular dynamics.” Optics Infobase Conference Papers, 2011. Scopus, doi:10.1364/ntm.2011.ntub3. Full Text

Pages