Adam P. Wax

Adam P. Wax

Professor of Biomedical Engineering

Office Location: 
2571 CIEMAS, Durham, NC 27708
Front Office Address: 
Box 90281, Durham, NC 27708-0281
Phone: 
(919) 660-5143

Overview

Dr. Wax's research interests include optical spectroscopy for early cancer detection, novel microscopy and
interferometry techniques.

The study of intact, living cells with optical spectroscopy offers the opportunity to observe cellular structure, organization and dynamics in a way that is not possible with traditional methods. We have developed a set of novel spectroscopic techniques for measuring spatial, temporal and refractive structure on sub-hertz and sub-wavelength scales based on using low-coherence interferometry (LCI) to detect scattered light. We have applied these techniques in different types of cell biology experiments. In one experiment, LCI measurements of the angular pattern of backscattered light are used to determine non-invasively the structure of sub-cellular organelles in cell monolayers, and the components of epithelial tissue from freshly excised rat esophagus. This work has potential as a diagnostic method for early cancer detection. In another experiment, LCI phase measurements are used to examine volume changes of epithelial cells in a monolayer in response to environmental osmolarity changes. Although cell volume changes have been measured previously, this work demonstrates for the first time the volume of just a few cells (2 or 3) tracked continuously and in situ.

Education & Training

  • Ph.D., Duke University 1999

  • M.A., Duke University 1996

  • B.S., Rensselaer Polytechnic Institute 1993

Selected Grants

Duke CTSA (KL2) awarded by National Institutes of Health (Mentor). 2018 to 2023

Duke CTSA (TL1) awarded by National Institutes of Health (Mentor). 2018 to 2023

Analysis of mechanical induction of bioelectric activity in cells awarded by Army Research Office (Principal Investigator). 2019 to 2022

Advanced a/LCI systems for improved clinical utility awarded by National Institutes of Health (Principal Investigator). 2016 to 2021

High throughput cell screening for toxic metal exposure awarded by National Institutes of Health (Principal Investigator). 2018 to 2020

High throughput cell screening for toxic metal exposure awarded by National Institutes of Health (Principal Investigator). 2018 to 2020

Novel Coherence Imaging to Evaluate the Health of the Cervical Epithelium awarded by National Institutes of Health (Principal Investigator). 2014 to 2020

Coherent light scattering for early detection of Alzheimer's disease awarded by National Institutes of Health (Principal Investigator). 2017 to 2020

Deep skin imaging with OCT awarded by National Science Foundation (Principal Investigator). 2018 to 2020

Visualization of mechanical stress in live cells awarded by National Science Foundation (Principal Investigator). 2016 to 2019

Pages

Capel, Blanche. Preface.. Vol. 134, 2019, pp. xiii–xvii. Pubmed, doi:10.1016/S0070-2153(19)30050-X. Full Text

Graf, R. N., et al. “Assessing microscopic structural features using fourier-domain low coherence interferometry.” Handbook of Biomedical Optics, 2016, pp. 463–82.

Wax, A., et al. “Elastic scattering spectroscopy and optical coherence tomography.” Optical Coherence Tomography: Technology and Applications, Second Edition, 2015, pp. 1207–35. Scopus, doi:10.1007/978-3-319-06419-2_38. Full Text

Vo-Dinh, T. Preface. 2014, pp. ix–xi. Scopus, doi:10.1201/b17289. Full Text

Wax, A., and F. E. Robles. “Molecular imaging true color spectroscopic (METRiCS) optical coherence tomography.” Medical Imaging: Technology and Applications, 2013, pp. 53–87. Scopus, doi:10.1201/b15511. Full Text

Zhang, Haoran, et al. “Angular range, sampling and noise considerations for inverse light scattering analysis of nuclear morphology..” Journal of Biophotonics, vol. 12, no. 2, Feb. 2019. Epmc, doi:10.1002/jbio.201800258. Full Text

Wax, A. “Oct promising as clear point-of-care solution.” Biophotonics International, vol. 25, no. 6, Sept. 2018, pp. 30–33.

Zhou, Y., et al. “Spectroscopic OCT: Towards an effective tool for distinguishing authentic and artificial Chinese freshwater pearls.” Optical Materials Express, vol. 8, no. 3, Mar. 2018, pp. 622–28. Scopus, doi:10.1364/OME.8.000622. Full Text

Kim, Sanghoon, et al. “Design and implementation of a low-cost, portable OCT system..” Biomedical Optics Express, vol. 9, no. 3, Mar. 2018, pp. 1232–43. Epmc, doi:10.1364/BOE.9.001232. Full Text

Muñoz, Alexandra, et al. “Cellular shear stiffness reflects progression of arsenic-induced transformation during G1..” Carcinogenesis, vol. 39, no. 2, Feb. 2018, pp. 109–17. Epmc, doi:10.1093/carcin/bgx116. Full Text

Steelman, Zachary A., et al. “Comparison of imaging fiber bundles for coherence-domain imaging..” Applied Optics, vol. 57, no. 6, Feb. 2018, pp. 1455–62. Epmc, doi:10.1364/ao.57.001455. Full Text

Zhao, Yang, et al. “Real-time speckle reduction in optical coherence tomography using the dual window method..” Biomedical Optics Express, vol. 9, no. 2, Feb. 2018, pp. 616–22. Epmc, doi:10.1364/BOE.9.000616. Full Text

Steelman, Zachary A., et al. “Is the nuclear refractive index lower than cytoplasm? Validation of phase measurements and implications for light scattering technologies..” Journal of Biophotonics, vol. 10, no. 12, Dec. 2017, pp. 1714–22. Epmc, doi:10.1002/jbio.201600314. Full Text

Chowdhury, Shwetadwip, et al. “Structured illumination microscopy for dual-modality 3D sub-diffraction resolution fluorescence and refractive-index reconstruction..” Biomedical Optics Express, vol. 8, no. 12, Dec. 2017, pp. 5776–93. Epmc, doi:10.1364/BOE.8.005776. Full Text

Pages

Jelly, E. T., et al. “Adaptability and performance of low-cost OCT System for use in Benchtop optical coherence microscopy.” Optics Infobase Conference Papers, vol. Part F91-TRANSLATIONAL 2018, 2018. Scopus, doi:10.1364/TRANSLATIONAL.2018.JW3A.32. Full Text

Ho, D., et al. “Scanning angle-resolved low coherence interferometry system for clinical detection of cervical dysplasia.” Optics Infobase Conference Papers, vol. Part F91-TRANSLATIONAL 2018, 2018. Scopus, doi:10.1364/TRANSLATIONAL.2018.CF4B.6. Full Text

Wax, A. “Development of a low-cost, portable optical coherence tomography (OCT) system.” Optics Infobase Conference Papers, vol. Part F96-AIO 2018, 2018. Scopus, doi:10.1364/AIO.2018.AW2A.4. Full Text

Song, G., et al. “Design and implementation of a low-cost, portable OCT system.” Optics Infobase Conference Papers, vol. Part F90-OTS 2018, 2018. Scopus, doi:10.1364/OTS.2018.OF3D.7. Full Text

Zhao, Y., et al. “Speckle reduction in optical coherence tomography at video rate.” Optics Infobase Conference Papers, vol. Part F90-OTS 2018, 2018. Scopus, doi:10.1364/OTS.2018.OF3D.3. Full Text

Zhao, Y., et al. “In vivo rat skin flap viability assessment using dual axis spectroscopic optical coherence tomography.” Optics Infobase Conference Papers, vol. Part F62-BODA 2017, 2017. Scopus, doi:10.1364/BODA.2017.BoM4A.4. Full Text

Wax, A. “Nanoscale phase imaging of live cells.” Optics Infobase Conference Papers, vol. Part F66-FiO 2017, 2017. Scopus, doi:10.1364/FIO.2017.FW6D.1. Full Text

Zhao, Y., et al. “Toward the assessment of blood oxygenation using multispectral multiple scattering low coherence interferometry.” Optics Infobase Conference Papers, 2016. Scopus, doi:10.1364/TRANSLATIONAL.2016.JM3A.24. Full Text

Kim, S., et al. “Guidance of angle-resolved low coherence interferometry using co-located optical coherence tomography on rat esophageal tissue.” Optics Infobase Conference Papers, 2016. Scopus, doi:10.1364/TRANSLATIONAL.2016.JTu3A.16. Full Text

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