Adam P. Wax
Professor of Biomedical Engineering
Professor of Physics (Secondary)
Faculty Network Member of the Duke Institute for Brain Sciences
Member of the Duke Cancer Institute
Dr. Wax's research interests include optical spectroscopy for early cancer detection, novel microscopy and
The study of intact, living cells with optical spectroscopy offers the opportunity to observe cellular structure, organization and dynamics in a way that is not possible with traditional methods. We have developed a set of novel spectroscopic techniques for measuring spatial, temporal and refractive structure on sub-hertz and sub-wavelength scales based on using low-coherence interferometry (LCI) to detect scattered light. We have applied these techniques in different types of cell biology experiments. In one experiment, LCI measurements of the angular pattern of backscattered light are used to determine non-invasively the structure of sub-cellular organelles in cell monolayers, and the components of epithelial tissue from freshly excised rat esophagus. This work has potential as a diagnostic method for early cancer detection. In another experiment, LCI phase measurements are used to examine volume changes of epithelial cells in a monolayer in response to environmental osmolarity changes. Although cell volume changes have been measured previously, this work demonstrates for the first time the volume of just a few cells (2 or 3) tracked continuously and in situ.
Coherence Imaging for Assessing Colorectal Neoplasia awarded by National Institutes of Health (Principal Investigator). 2009 to 2012
Molecular Imaging Using Hyperspectral Darkfield Microscope of Nanoparticles awarded by National Science Foundation (Principal Investigator). 2007 to 2011
Assessing Deployment of Microbicidal Gels With Label-Free Optical Measurement awarded by National Institutes of Health (Principal Investigator). 2007 to 2010
In Vivo Detection of Pre-Cancerous Lesions Using a /LCI awarded by National Institutes of Health (Principal Investigator). 2004 to 2010
Low Coherence Light Scattering for Biophotonics awarded by National Science Foundation (Principal Investigator). 2005 to 2010
CAREER: Low Coherence Light Scattering for Biophotonics awarded by National Science Foundation (Principal Investigator). 2004 to 2009
Assessing Nuclear Morphology in Thick Tissues Using fLCI awarded by National Institutes of Health (Principal Investigator). 2006 to 2008
In Vivo Detection of Pre-Cancerous Lesions Using a/LCI awarded by National Institutes of Health (Principal Investigator). 2004 to 2006
Rinehart, Matthew T., et al. “Hemoglobin consumption by P. falciparum in individual erythrocytes imaged via quantitative phase spectroscopy.” Sci Rep, vol. 6, Apr. 2016, p. 24461. Pubmed, doi:10.1038/srep24461. Full Text
Kim, Sanghoon, et al. “Analyzing spatial correlations in tissue using angle-resolved low coherence interferometry measurements guided by co-located optical coherence tomography.” Biomed Opt Express, vol. 7, no. 4, Apr. 2016, pp. 1400–14. Pubmed, doi:10.1364/BOE.7.001400. Full Text
Eldridge, Will J., et al. “Imaging deformation of adherent cells due to shear stress using quantitative phase imaging.” Optics Letters, vol. 41, no. 2, Jan. 2016, pp. 352–55. Epmc, doi:10.1364/ol.41.000352. Full Text
Park, Han Sang, et al. “Automated Detection of P. falciparum Using Machine Learning Algorithms with Quantitative Phase Images of Unstained Cells.” Plos One, vol. 11, no. 9, 2016, p. e0163045. Pubmed, doi:10.1371/journal.pone.0163045. Full Text Open Access Copy
Chowdhury, Shwetadwip, et al. “Spatial frequency-domain multiplexed microscopy for simultaneous, single-camera, one-shot, fluorescent, and quantitative-phase imaging.” Optics Letters, vol. 40, no. 21, Nov. 2015, pp. 4839–42. Epmc, doi:10.1364/ol.40.004839. Full Text
Zhao, Yang, et al. “Evaluation of burn severity in vivo in a mouse model using spectroscopic optical coherence tomography.” Biomed Opt Express, vol. 6, no. 9, Sept. 2015, pp. 3339–45. Pubmed, doi:10.1364/BOE.6.003339. Full Text
Ho, Derek, et al. “Evaluation of hybrid algorithm for analysis of scattered light using ex vivo nuclear morphology measurements of cervical epithelium.” Biomed Opt Express, vol. 6, no. 8, Aug. 2015, pp. 2755–65. Pubmed, doi:10.1364/BOE.6.002755. Full Text
Sheinfeld, Adi, et al. “Quantitative phase imaging with molecular sensitivity using photoacoustic microscopy with a miniature ring transducer.” Journal of Biomedical Optics, vol. 20, no. 8, Aug. 2015, p. 86002. Epmc, doi:10.1117/1.jbo.20.8.086002. Full Text
Maher, Jason R., et al. “Co-localized confocal Raman spectroscopy and optical coherence tomography (CRS-OCT) for depth-resolved analyte detection in tissue.” Biomedical Optics Express, vol. 6, no. 6, June 2015, pp. 2022–35. Epmc, doi:10.1364/boe.6.002022. Full Text
Rinehart, Matthew T., et al. “Influence of defocus on quantitative analysis of microscopic objects and individual cells with digital holography.” Biomedical Optics Express, vol. 6, no. 6, June 2015, pp. 2067–75. Epmc, doi:10.1364/boe.6.002067. Full Text
Rinehart, M. T., et al. “Whole-cell analysis of cardiomyocytes with combined quantitative phase and two-channel fluorescence microscopy.” Optics Infobase Conference Papers, 2011.
Zhu, Y., et al. “Spectral-domain differential interference contrast microscopy.” Optics Infobase Conference Papers, 2011.
Rinehart, M., et al. “Quantitative phase microscopy with multi-wavelength unwrapping and tomographic 3d reconstruction.” Biomedical Optics, Biomed 2008, 2008.