Adam P. Wax
Professor of Biomedical Engineering
Professor of Physics (Secondary)
Faculty Network Member of the Duke Institute for Brain Sciences
Member of the Duke Cancer Institute
Dr. Wax's research interests include optical spectroscopy for early cancer detection, novel microscopy and
The study of intact, living cells with optical spectroscopy offers the opportunity to observe cellular structure, organization and dynamics in a way that is not possible with traditional methods. We have developed a set of novel spectroscopic techniques for measuring spatial, temporal and refractive structure on sub-hertz and sub-wavelength scales based on using low-coherence interferometry (LCI) to detect scattered light. We have applied these techniques in different types of cell biology experiments. In one experiment, LCI measurements of the angular pattern of backscattered light are used to determine non-invasively the structure of sub-cellular organelles in cell monolayers, and the components of epithelial tissue from freshly excised rat esophagus. This work has potential as a diagnostic method for early cancer detection. In another experiment, LCI phase measurements are used to examine volume changes of epithelial cells in a monolayer in response to environmental osmolarity changes. Although cell volume changes have been measured previously, this work demonstrates for the first time the volume of just a few cells (2 or 3) tracked continuously and in situ.
Terry, Neil G., et al. “Detection of dysplasia in Barrett's esophagus with in vivo depth-resolved nuclear morphology measurements.” Gastroenterology, vol. 140, no. 1, Jan. 2011, pp. 42–50. Epmc, doi:10.1053/j.gastro.2010.09.008. Full Text
Zhu, Yizheng, et al. “Development of angle-resolved low coherence interferometry for clinical detection of dysplasia.” Journal of Carcinogenesis, vol. 10, Jan. 2011, p. 19. Epmc, doi:10.4103/1477-3163.83935. Full Text
Skala, M. C., et al. “Photothermal optical coherence tomography for molecular imaging.” Optics Infobase Conference Papers, 2011.
Rinehart, M. T., et al. “Whole-cell analysis of cardiomyocytes with combined quantitative phase and two-channel fluorescence microscopy.” Optics Infobase Conference Papers, 2011.
Wax, A. “Coherence imaging.” Optics Infobase Conference Papers, Dec. 2010.
Chowdhury, S., et al. “Detecting hemoglobin concentration using the dual window method for processing spectroscopic optical coherence tomography signals.” Optics Infobase Conference Papers, Dec. 2010.
Shaked, N. T., and A. Wax. “New directions in interferometric phase microscopy of biological cell dynamics.” Optics Infobase Conference Papers, Dec. 2010.
Robles, Francisco E., and Adam Wax. “Separating the scattering and absorption coefficients using the real and imaginary parts of the refractive index with low-coherence interferometry.” Optics Letters, vol. 35, no. 17, Sept. 2010, pp. 2843–45. Epmc, doi:10.1364/ol.35.002843. Full Text Open Access Copy
Robles, Francisco E., et al. “Detection of early colorectal cancer development in the azoxymethane rat carcinogenesis model with Fourier domain low coherence interferometry.” Biomedical Optics Express, vol. 1, no. 2, Aug. 2010, pp. 736–45. Epmc, doi:10.1364/boe.1.000736. Full Text
Shaked, Natan T., et al. “Whole-cell-analysis of live cardiomyocytes using wide-field interferometric phase microscopy.” Biomedical Optics Express, vol. 1, no. 2, Aug. 2010, pp. 706–19. Epmc, doi:10.1364/boe.1.000706. Full Text Open Access Copy