Adam P. Wax
Professor of Biomedical Engineering
Professor of Physics (Secondary)
Faculty Network Member of the Duke Institute for Brain Sciences
Member of the Duke Cancer Institute
Dr. Wax's research interests include optical spectroscopy for early cancer detection, novel microscopy and
The study of intact, living cells with optical spectroscopy offers the opportunity to observe cellular structure, organization and dynamics in a way that is not possible with traditional methods. We have developed a set of novel spectroscopic techniques for measuring spatial, temporal and refractive structure on sub-hertz and sub-wavelength scales based on using low-coherence interferometry (LCI) to detect scattered light. We have applied these techniques in different types of cell biology experiments. In one experiment, LCI measurements of the angular pattern of backscattered light are used to determine non-invasively the structure of sub-cellular organelles in cell monolayers, and the components of epithelial tissue from freshly excised rat esophagus. This work has potential as a diagnostic method for early cancer detection. In another experiment, LCI phase measurements are used to examine volume changes of epithelial cells in a monolayer in response to environmental osmolarity changes. Although cell volume changes have been measured previously, this work demonstrates for the first time the volume of just a few cells (2 or 3) tracked continuously and in situ.
Improving Medical Outcomes Through Needle-Based Optical Sensing awarded by (Principal Investigator). 2015 to 2017
Novel Determination of Microbicide PK in Women's Reproductive Health awarded by National Institutes of Health (Co Investigator). 2012 to 2017
EAGER: Cell phone enabled spectroscopy awarded by National Science Foundation (Principal Investigator). 2014 to 2016
Coherent light scattering for early detection of retinal disease awarded by National Institutes of Health (Principal Investigator). 2013 to 2016
Inhibition of Reflux-Induced Esophageal Adenocarcinoma by Proanthocyanidins awarded by Medical College of Wisconsin (Principal Investigator). 2013 to 2016
InCh Microscope: Compact and Portable Quantitative Phase Microscope for Label-Free Morphological Diagnosis of Blood Samp awarded by (Principal Investigator). 2014
Increased Depth Penetration in Coherence Imaging Using Multiply Scattered Light awarded by National Science Foundation (Principal Investigator). 2011 to 2014
MRI: Development of a Hybrid Quantitative Phase Microscope for Live Cell Imaging awarded by National Science Foundation (Principal Investigator). 2010 to 2014
Cross-disciplinary Training in Medical Physics awarded by National Institutes of Health (Mentor). 2007 to 2013
I-Corps: InCh holographic microscope for cell diagnostics awarded by National Science Foundation (Principal Investigator). 2012
Zhao, Yang, et al. “Dual-axis optical coherence tomography for deep tissue imaging.” Opt Lett, vol. 42, no. 12, June 2017, pp. 2302–05. Pubmed, doi:10.1364/OL.42.002302. Full Text
Chowdhury, S., et al. “Refractive index tomography with structured illumination.” Optica, vol. 4, no. 5, May 2017, pp. 537–45. Scopus, doi:10.1364/OPTICA.4.000537. Full Text
Chowdhury, Shwetadwip, et al. “Structured illumination multimodal 3D-resolved quantitative phase and fluorescence sub-diffraction microscopy.” Biomedical Optics Express, vol. 8, no. 5, May 2017, pp. 2496–518. Epmc, doi:10.1364/boe.8.002496. Full Text
Ho, Derek, et al. “Feasibility of clinical detection of cervical dysplasia using angle-resolved low coherence interferometry measurements of depth-resolved nuclear morphology.” International Journal of Cancer, vol. 140, no. 6, Mar. 2017, pp. 1447–56. Epmc, doi:10.1002/ijc.30539. Full Text
Eldridge, Will J., et al. “Optical Phase Measurements of Disorder Strength Link Microstructure to Cell Stiffness.” Biophysical Journal, vol. 112, no. 4, Feb. 2017, pp. 692–702. Epmc, doi:10.1016/j.bpj.2016.12.016. Full Text
Chuchuen, Oranat, et al. “Label-Free Measurements of Tenofovir Diffusion Coefficients in a Microbicide Gel Using Raman Spectroscopy.” Journal of Pharmaceutical Sciences, vol. 106, no. 2, Feb. 2017, pp. 639–44. Epmc, doi:10.1016/j.xphs.2016.09.030. Full Text
Chuchuen, Oranat, et al. “Label-free analysis of tenofovir delivery to vaginal tissue using co-registered confocal Raman spectroscopy and optical coherence tomography.” Plos One, vol. 12, no. 9, Jan. 2017, p. e0185633. Epmc, doi:10.1371/journal.pone.0185633. Full Text
Zhao, Yang, et al. “Deep imaging of absorption and scattering features by multispectral multiple scattering low coherence interferometry.” Biomed Opt Express, vol. 7, no. 10, Oct. 2016, pp. 3916–26. Pubmed, doi:10.1364/BOE.7.003916. Full Text
Wax, A., and K. Chu. “Found in translation: Biophotonics from lab to clinic.” Optics and Photonics News, vol. 27, no. 9, Sept. 2016, pp. 34–41. Scopus, doi:10.1364/OPN.27.9.000034. Full Text
Zhao, Y., et al. “Toward the assessment of blood oxygenation using multispectral multiple scattering low coherence interferometry.” Optics Infobase Conference Papers, 2016.
Wax, A. “Molecular contrast in interferometric imaging.” Conference on Lasers and Electro Optics Europe Technical Digest, vol. 2015-August, 2015.
Ho, D., et al. “Clinical detection of cervical dysplasia using angle-resolved low coherence interferometry.” Optics Infobase Conference Papers, 2014. Scopus, doi:10.1364/CANCER.2016.CTh4A.2. Full Text
Steelman, Z. A., et al. “Corruption of refractive index measurements of spheres using quantitative phase microscopy.” Optics Infobase Conference Papers, 2014. Scopus, doi:10.1364/FIO.2016.JTh2A.126. Full Text
Zhao, Y., et al. “Toward the assessment of blood oxygenation using multispectral multiple scattering low coherence interferometry.” Optics Infobase Conference Papers, 2014. Scopus, doi:10.1364/CANCER.2016.JM3A.24. Full Text
Eldridge, W. J., et al. “Linking cellular disorder strength and shear stiffness using quantitative phase imaging.” Optics Infobase Conference Papers, 2014. Scopus, doi:10.1364/FIO.2016.FF3A.6. Full Text
Kim, S., et al. “Guidance of angle-resolved low coherence interferometry using co-located optical coherence tomography on rat esophageal tissue.” Optics Infobase Conference Papers, 2014. Scopus, doi:10.1364/CANCER.2016.JTu3A.16. Full Text
Shaked, N. T., et al. “Dynamic quantitative microscopy and nanoscopy of red blood cells in sickle cell disease.” Progress in Biomedical Optics and Imaging Proceedings of Spie, vol. 8227, 2012. Scopus, doi:10.1117/12.907659. Full Text
Skala, M. C., et al. “Photothermal optical coherence tomography for molecular imaging.” Optics Infobase Conference Papers, 2011.
Rinehart, M. T., et al. “Real-time quantitative phase and dual-channel fluorescence microscopy for studying cellular and biomolecular dynamics.” Optics Infobase Conference Papers, 2011.