Adam P. Wax
Professor of Biomedical Engineering
Professor of Physics (Secondary)
Faculty Network Member of the Duke Institute for Brain Sciences
Member of the Duke Cancer Institute
Dr. Wax's research interests include optical spectroscopy for early cancer detection, novel microscopy and
The study of intact, living cells with optical spectroscopy offers the opportunity to observe cellular structure, organization and dynamics in a way that is not possible with traditional methods. We have developed a set of novel spectroscopic techniques for measuring spatial, temporal and refractive structure on sub-hertz and sub-wavelength scales based on using low-coherence interferometry (LCI) to detect scattered light. We have applied these techniques in different types of cell biology experiments. In one experiment, LCI measurements of the angular pattern of backscattered light are used to determine non-invasively the structure of sub-cellular organelles in cell monolayers, and the components of epithelial tissue from freshly excised rat esophagus. This work has potential as a diagnostic method for early cancer detection. In another experiment, LCI phase measurements are used to examine volume changes of epithelial cells in a monolayer in response to environmental osmolarity changes. Although cell volume changes have been measured previously, this work demonstrates for the first time the volume of just a few cells (2 or 3) tracked continuously and in situ.
Shaked, Natan T., et al. “Quantitative microscopy and nanoscopy of sickle red blood cells performed by wide field digital interferometry.” J Biomed Opt, vol. 16, no. 3, Mar. 2011, p. 030506. Pubmed, doi:10.1117/1.3556717. Full Text
Crow, Matthew J., et al. “Polarization mapping of nanoparticle plasmonic coupling.” Optics Letters, vol. 36, no. 5, Mar. 2011, pp. 757–59. Epmc, doi:10.1364/ol.36.000757. Full Text
Zhu, Yizheng, et al. “Spectral-domain differential interference contrast microscopy.” Optics Letters, vol. 36, no. 4, Feb. 2011, pp. 430–32. Epmc, doi:10.1364/ol.36.000430. Full Text
Giacomelli, Michael G., and Adam Wax. “Imaging beyond the ballistic limit in coherence imaging using multiply scattered light.” Optics Express, vol. 19, no. 5, Feb. 2011, pp. 4268–79. Epmc, doi:10.1364/oe.19.004268. Full Text
Zhu, Yizheng, et al. “Design and validation of an angle-resolved low-coherence interferometry fiber probe for in vivo clinical measurements of depth-resolved nuclear morphology.” Journal of Biomedical Optics, vol. 16, no. 1, Jan. 2011, p. 011003. Epmc, doi:10.1117/1.3520130. Full Text
Terry, Neil G., et al. “Detection of dysplasia in Barrett's esophagus with in vivo depth-resolved nuclear morphology measurements.” Gastroenterology, vol. 140, no. 1, Jan. 2011, pp. 42–50. Epmc, doi:10.1053/j.gastro.2010.09.008. Full Text
Zhu, Yizheng, et al. “Development of angle-resolved low coherence interferometry for clinical detection of dysplasia.” Journal of Carcinogenesis, vol. 10, Jan. 2011, p. 19. Epmc, doi:10.4103/1477-3163.83935. Full Text
Skala, M. C., et al. “Photothermal optical coherence tomography for molecular imaging.” Optics Infobase Conference Papers, 2011.
Rinehart, M. T., et al. “Whole-cell analysis of cardiomyocytes with combined quantitative phase and two-channel fluorescence microscopy.” Optics Infobase Conference Papers, 2011.
Zhu, Y., et al. “Spectral-domain differential interference contrast microscopy.” Optics Infobase Conference Papers, 2011.