Adam P. Wax
Professor of Biomedical Engineering
Professor of Physics (Secondary)
Faculty Network Member of the Duke Institute for Brain Sciences
Member of the Duke Cancer Institute
Dr. Wax's research interests include optical spectroscopy for early cancer detection, novel microscopy and
The study of intact, living cells with optical spectroscopy offers the opportunity to observe cellular structure, organization and dynamics in a way that is not possible with traditional methods. We have developed a set of novel spectroscopic techniques for measuring spatial, temporal and refractive structure on sub-hertz and sub-wavelength scales based on using low-coherence interferometry (LCI) to detect scattered light. We have applied these techniques in different types of cell biology experiments. In one experiment, LCI measurements of the angular pattern of backscattered light are used to determine non-invasively the structure of sub-cellular organelles in cell monolayers, and the components of epithelial tissue from freshly excised rat esophagus. This work has potential as a diagnostic method for early cancer detection. In another experiment, LCI phase measurements are used to examine volume changes of epithelial cells in a monolayer in response to environmental osmolarity changes. Although cell volume changes have been measured previously, this work demonstrates for the first time the volume of just a few cells (2 or 3) tracked continuously and in situ.
Seekell, Kevin, et al. “Optimization of immunolabeled plasmonic nanoparticles for cell surface receptor analysis..” Methods (San Diego, Calif.), vol. 56, no. 2, Feb. 2012, pp. 310–16. Epmc, doi:10.1016/j.ymeth.2011.08.017. Full Text
Zhu, Yizheng, et al. “Angle-resolved low-coherence interferometry: an optical biopsy technique for clinical detection of dysplasia in Barrett's esophagus..” Expert Review of Gastroenterology & Hepatology, vol. 6, no. 1, Feb. 2012, pp. 37–41. Epmc, doi:10.1586/egh.11.83. Full Text
Matthews, Thomas E., and Adam Wax. “High-efficiency diffuse Raman spectroscopy through a fiber bundle..” Conference Proceedings : ... Annual International Conference of the Ieee Engineering in Medicine and Biology Society. Ieee Engineering in Medicine and Biology Society. Annual Conference, vol. 2012, Jan. 2012, pp. 1181–83. Epmc, doi:10.1109/embc.2012.6346147. Full Text
Rinehart, Matthew T., et al. “Time-resolved imaging refractometry of microbicidal films using quantitative phase microscopy..” Journal of Biomedical Optics, vol. 16, no. 12, Dec. 2011. Epmc, doi:10.1117/1.3665439. Full Text
Robles, Francisco E., et al. “Molecular imaging true-colour spectroscopic optical coherence tomography..” Nature Photonics, vol. 5, no. 12, Dec. 2011, pp. 744–47. Epmc, doi:10.1038/nphoton.2011.257. Full Text
Seekell, K., et al. “Controlled synthesis of gold nanorods and application to brain tumor delineation.” Optics Infobase Conference Papers, Dec. 2011.
Shaked, N. T., et al. “Quantitative phase microscopy of biological cell dynamics by wide-field digital interferometry.” Springer Series in Surface Sciences, vol. 46, no. 1, Dec. 2011, pp. 169–98. Scopus, doi:10.1007/978-3-642-15813-1_7. Full Text
Seekell, Kevin, et al. “Hyperspectral molecular imaging of multiple receptors using immunolabeled plasmonic nanoparticles..” Journal of Biomedical Optics, vol. 16, no. 11, Nov. 2011. Epmc, doi:10.1117/1.3646529. Full Text