Adam P. Wax
Professor of Biomedical Engineering
Professor of Physics (Secondary)
Faculty Network Member of the Duke Institute for Brain Sciences
Member of the Duke Cancer Institute
Dr. Wax's research interests include optical spectroscopy for early cancer detection, novel microscopy and
The study of intact, living cells with optical spectroscopy offers the opportunity to observe cellular structure, organization and dynamics in a way that is not possible with traditional methods. We have developed a set of novel spectroscopic techniques for measuring spatial, temporal and refractive structure on sub-hertz and sub-wavelength scales based on using low-coherence interferometry (LCI) to detect scattered light. We have applied these techniques in different types of cell biology experiments. In one experiment, LCI measurements of the angular pattern of backscattered light are used to determine non-invasively the structure of sub-cellular organelles in cell monolayers, and the components of epithelial tissue from freshly excised rat esophagus. This work has potential as a diagnostic method for early cancer detection. In another experiment, LCI phase measurements are used to examine volume changes of epithelial cells in a monolayer in response to environmental osmolarity changes. Although cell volume changes have been measured previously, this work demonstrates for the first time the volume of just a few cells (2 or 3) tracked continuously and in situ.
Wax, Adam, et al. “Comparative review of interferometric detection of plasmonic nanoparticles.” Biomedical Optics Express, vol. 4, no. 10, Jan. 2013, pp. 2166–78. Epmc, doi:10.1364/boe.4.002166. Full Text
Wax, A. “Wigner distribution: Friend to biophotonics.” Optics Infobase Conference Papers, Jan. 2013.
Drake, Tyler K., et al. “Measuring dilution of microbicide gels with optical imaging.” Plos One, vol. 8, no. 12, Jan. 2013, p. e82213. Epmc, doi:10.1371/journal.pone.0082213. Full Text
Seekell, Kevin, et al. “Feasibility study of brain tumor delineation using immunolabeled gold nanorods.” Biomedical Optics Express, vol. 4, no. 11, Jan. 2013, pp. 2284–95. Epmc, doi:10.1364/boe.4.002284. Full Text
Yarmoska, Steven K., et al. “A scattering phantom for observing long range order with two-dimensional angle-resolved Low-Coherence Interferometry.” Biomedical Optics Express, vol. 4, no. 9, Jan. 2013, pp. 1742–48. Epmc, doi:10.1364/boe.4.001742. Full Text
Wax, Adam, and Kevin J. Chalut. “Nuclear morphology measurements with angle-resolved low coherence interferometry for application to cell biology and early cancer detection.” Studies in Health Technology and Informatics, vol. 185, Jan. 2013, pp. 129–51.
Wax, A. “Coherence imaging for early cancer detection.” 2012 Conference on Lasers and Electro Optics, Cleo 2012, Dec. 2012.
Kim, S., et al. “Spectrally multiplexed photothermal OCT and novel detection methods.” 2012 Conference on Lasers and Electro Optics, Cleo 2012, Dec. 2012.
Li, Y., et al. “Assessing nanoparticle concentration using METRiCS optical coherence tomography.” Biomedical Optics, Biomed 2012, Dec. 2012.
Zhu, Y., et al. “Spectral-domain differential interference contrast (SD-DIC) microscopy for measuring live cell dynamics.” Biomedical Optics, Biomed 2012, Dec. 2012.