Adam P. Wax
Professor of Biomedical Engineering
Professor of Physics (Secondary)
Faculty Network Member of the Duke Institute for Brain Sciences
Member of the Duke Cancer Institute
Dr. Wax's research interests include optical spectroscopy for early cancer detection, novel microscopy and
The study of intact, living cells with optical spectroscopy offers the opportunity to observe cellular structure, organization and dynamics in a way that is not possible with traditional methods. We have developed a set of novel spectroscopic techniques for measuring spatial, temporal and refractive structure on sub-hertz and sub-wavelength scales based on using low-coherence interferometry (LCI) to detect scattered light. We have applied these techniques in different types of cell biology experiments. In one experiment, LCI measurements of the angular pattern of backscattered light are used to determine non-invasively the structure of sub-cellular organelles in cell monolayers, and the components of epithelial tissue from freshly excised rat esophagus. This work has potential as a diagnostic method for early cancer detection. In another experiment, LCI phase measurements are used to examine volume changes of epithelial cells in a monolayer in response to environmental osmolarity changes. Although cell volume changes have been measured previously, this work demonstrates for the first time the volume of just a few cells (2 or 3) tracked continuously and in situ.
Curry, A., et al. “Substrate effect on refractive index dependence of plasmon resonance for individual silver nanoparticles observed using darkfield microspectroscopy.” Optics Express, vol. 13, no. 7, Apr. 2005, pp. 2668–77. Epmc, doi:10.1364/opex.13.002668. Full Text
Pyhtila, John W., and Adam Wax. “Improved interferometric detection of scattered light with a 4f imaging system.” Applied Optics, vol. 44, no. 10, Apr. 2005, pp. 1785–91. Epmc, doi:10.1364/ao.44.001785. Full Text
Ahn, Andrew, et al. “Harmonic phase-dispersion microscope with a Mach-Zehnder interferometer.” Applied Optics, vol. 44, no. 7, Mar. 2005, pp. 1188–90. Epmc, doi:10.1364/ao.44.001188. Full Text
Wax, Adam. “Low-coherence light-scattering calculations for polydisperse size distributions.” Journal of the Optical Society of America. A, Optics, Image Science, and Vision, vol. 22, no. 2, Feb. 2005, pp. 256–61. Epmc, doi:10.1364/josaa.22.000256. Full Text
Curry, A., et al. “Measurement system for the high-throughput characterization of metal nanoparticles for biosensors.” Optics Infobase Conference Papers, Jan. 2005.
Chou, D. R., and A. P. Wax. “Optical scattering of confocal laser scanning reflectance microscopy in turbid media.” Optics Infobase Conference Papers, Jan. 2005.
Pyhtila, John W., and Adam Wax. “Rapid, depth-resolved light scattering measurements using Fourier domain, angle-resolved low coherence interferometry.” Optics Express, vol. 12, no. 25, Dec. 2004, pp. 6178–83. Epmc, doi:10.1364/opex.12.006178. Full Text
Iwai, Hidenao, et al. “Quantitative phase imaging using actively stabilized phase-shifting low-coherence interferometry.” Optics Letters, vol. 29, no. 20, Oct. 2004, pp. 2399–401. Epmc, doi:10.1364/ol.29.002399. Full Text
Tunnell, J. W., et al. “Instrumentation for multi-modal spectroscopic diagnosis of epithelial dysplasia.” Technology in Cancer Research & Treatment, vol. 2, no. 6, Dec. 2003, pp. 505–14. Epmc, doi:10.1177/153303460300200603. Full Text
Pyhtila, John, et al. “Determining nuclear morphology using an improved angle-resolved low coherence interferometry system.” Optics Express, vol. 11, no. 25, Dec. 2003, pp. 3473–84. Epmc, doi:10.1364/oe.11.003473. Full Text