Nicolas Buchler

Nicolas Buchler

Assistant Professor of Biology

Office Location: 
Rm 2353a, CIEMAS Bldg, 101 Science Drive, Duke Box 3382, Durham, NC 27710
Front Office Address: 
Box 90338, 137 Bio Sci, Durham, NC 27708
(919) 660-5701


Our lab is interested in the systems biology and evolution of epigenetic switches (bistability) and clocks (oscillators) in gene regulatory networks, two functions that are essential for patterning, cell proliferation, and differentiation in biological systems. We also study biochemical oscillators such as the cell cycle, metabolic rhythms, and circadian clocks, which co-exist in the same cells and interact with one another through shared resources.

Education & Training

  • Ph.D., University of Michigan at Ann Arbor 2001

  • M.S., University of Michigan at Ann Arbor 1998

  • B.S., University of California at San Diego 1995

Selected Grants

Summer Scholars Program in Genome Sciences and Medicine awarded by National Institutes of Health (Significant Contributor). 2017 to 2022

Training Program in Developmental and Stem Cell Biology awarded by National Institutes of Health (Mentor). 2001 to 2022

Measuring and perturbing metabolic rhythms and the cell division cycle in single cells awarded by National Institutes of Health (Principal Investigator). 2018 to 2022

Bioinformatics and Computational Biology Training Program awarded by National Institutes of Health (Mentor). 2005 to 2021

Determining mechanisms of rewiring of the eukaryotic cell cycle by a virus without disrupting network function awarded by National Science Foundation (Principal Investigator). 2018 to 2021

Genetics Training Grant awarded by National Institutes of Health (Mentor). 1979 to 2020

Organization and Function of Cellular Structure awarded by National Institutes of Health (Mentor). 1975 to 2020

Molecular Mycology and Pathogenesis Training Program awarded by National Institutes of Health (Mentor). 2002 to 2019

Rewiring the yeast brain: Redundancy and interference in genetic networks awarded by National Institutes of Health (Principal Investigator). 2011 to 2016

Structural Biology and Biophysics Training Program awarded by National Institutes of Health (Mentor). 1994 to 2015

Hendler, A, Medina, EM, Buchler, NE, de Bruin, RAM, and Aharoni, A. "The evolution of a G1/S transcriptional network in yeasts." Current genetics 64.1 (February 2018): 81-86. (Review) Full Text

Lin, YT, and Buchler, NE. "Efficient analysis of stochastic gene dynamics in the non-adiabatic regime using piecewise deterministic Markov processes." Journal of the Royal Society, Interface 15.138 (January 2018). Full Text

Gómez-Schiavon, M, Chen, L-F, West, AE, and Buchler, NE. "BayFish: Bayesian inference of transcription dynamics from population snapshots of single-molecule RNA FISH in single cells." Genome biology 18.1 (September 4, 2017): 164-. Full Text

Hendler, A, Medina, EM, Kishkevich, A, Abu-Qarn, M, Klier, S, Buchler, NE, de Bruin, RAM, and Aharoni, A. "Gene duplication and co-evolution of G1/S transcription factor specificity in fungi are essential for optimizing cell fitness." PLoS genetics 13.5 (May 15, 2017): e1006778-. Full Text

Liban, TJ, Medina, EM, Tripathi, S, Sengupta, S, Henry, RW, Buchler, NE, and Rubin, SM. "Conservation and divergence of C-terminal domain structure in the retinoblastoma protein family." Proceedings of the National Academy of Sciences of the United States of America 114.19 (May 2017): 4942-4947. Full Text

Tanouchi, Y, Pai, A, Park, H, Huang, S, Buchler, NE, and You, L. "Long-term growth data of Escherichia coli at a single-cell level." Scientific data 4 (March 28, 2017): 170036-. Full Text

Medina, EM, Turner, JJ, Gordân, R, Skotheim, JM, and Buchler, NE. "Punctuated evolution and transitional hybrid network in an ancestral cell cycle of fungi." eLife 5 (May 10, 2016). Full Text Open Access Copy

Burnetti, AJ, Aydin, M, and Buchler, NE. "Cell cycle Start is coupled to entry into the yeast metabolic cycle across diverse strains and growth rates." Molecular biology of the cell 27.1 (January 2016): 64-74. Full Text Open Access Copy

Karapetyan, S, and Buchler, NE. "Role of DNA binding sites and slow unbinding kinetics in titration-based oscillators." Physical review. E, Statistical, nonlinear, and soft matter physics 92.6 (December 22, 2015): 062712-. Full Text Open Access Copy

Schaap, P, Barrantes, I, Minx, P, Sasaki, N, Anderson, RW, Bénard, M, Biggar, KK, Buchler, NE, Bundschuh, R, Chen, X, Fronick, C, Fulton, L, Golderer, G, Jahn, N, Knoop, V, Landweber, LF, Maric, C, Miller, D, Noegel, AA, Peace, R, Pierron, G, Sasaki, T, Schallenberg-Rüdinger, M, Schleicher, M, Singh, R, Spaller, T, Storey, KB, Suzuki, T, Tomlinson, C, Tyson, JJ, Warren, WC, Werner, ER, Werner-Felmayer, G, Wilson, RK, Winckler, T, Gott, JM, Glöckner, G, and Marwan, W. "The Physarum polycephalum Genome Reveals Extensive Use of Prokaryotic Two-Component and Metazoan-Type Tyrosine Kinase Signaling." Genome biology and evolution 8.1 (November 27, 2015): 109-125. Full Text Open Access Copy