Nicolas Buchler

Nicolas Buchler

Assistant Professor of Biology

Office Location: 
Rm 2353a, CIEMAS Bldg, 101 Science Drive, Duke Box 3382, Durham, NC 27710
Front Office Address: 
Box 90338, 137 Bio Sci, Durham, NC 27708
(919) 660-5701


Our lab is interested in the systems biology and evolution of epigenetic switches (bistability) and clocks (oscillators) in gene regulatory networks, two functions that are essential for patterning, cell proliferation, and differentiation in biological systems. We also study biochemical oscillators such as the cell cycle, metabolic rhythms, and circadian clocks, which co-exist in the same cells and interact with one another through shared resources.

Education & Training

  • Ph.D., University of Michigan at Ann Arbor 2001

  • M.S., University of Michigan at Ann Arbor 1998

  • B.S., University of California at San Diego 1995

Rienzo, A, Poveda-Huertes, D, Aydin, S, Buchler, NE, Pascual-Ahuir, A, and Proft, M. "Different Mechanisms Confer Gradual Control and Memory at Nutrient- and Stress-Regulated Genes in Yeast." Molecular and cellular biology 35.21 (November 2015): 3669-3683. Full Text Open Access Copy

Tanouchi, Y, Pai, A, Park, H, Huang, S, Stamatov, R, Buchler, NE, and You, L. "A noisy linear map underlies oscillations in cell size and gene expression in bacteria." Nature 523.7560 (July 2015): 357-360. Full Text Open Access Copy

Zhou, M, Wang, W, Karapetyan, S, Mwimba, M, Marqués, J, Buchler, NE, and Dong, X. "Redox rhythm reinforces the circadian clock to gate immune response." Nature 523.7561 (July 2015): 472-476. Full Text Open Access Copy

Mazo-Vargas, A, Park, H, Aydin, M, and Buchler, NE. "Measuring fast gene dynamics in single cells with time-lapse luminescence microscopy." Molecular biology of the cell 25.22 (November 2014): 3699-3708. Full Text Open Access Copy

Tanouchi, Y, Pai, A, Buchler, NE, and You, L. "Programming stress-induced altruistic death in engineered bacteria." Mol Syst Biol 8 (2012): 626-. Full Text Open Access Copy

Buchler, NE, and Bai, L. "Chromatin: Bind at your own RSC." Current Biology 21.6 (2011): R223-R225. Full Text Open Access Copy

Cross, FR, Buchler, NE, and Skotheim, JM. "Evolution of networks and sequences in eukaryotic cell cycle control." Philosophical Transactions of the Royal Society B: Biological Sciences 366.1584 (2011): 3532-3544. Full Text Open Access Copy

Buchler, NE, and Cross, FR. "Protein sequestration generates a flexible ultrasensitive response in a genetic network." Molecular Systems Biology 5 (2009). Full Text

Buchler, NE, and Louis, M. "Molecular Titration and Ultrasensitivity in Regulatory Networks." Journal of Molecular Biology 384.5 (2008): 1106-1119. Full Text

Fritz, G, Buchler, NE, Hwa, T, and Gerland, U. "Designing sequential transcription logic: A simple genetic circuit for conditional memory." Systems and Synthetic Biology 1.2 (2007): 89-98. Full Text