G. Allan Johnson

G. Allan Johnson

Charles E. Putman University Professor of Radiology

Office Location: 
141D Bryan Neuroscience Bldg, Research Drive, Durham, NC 27710
Front Office Address: 
Box 3302 Med Ctr, Durham, NC 27710
(919) 684-7754
Office Hours: 
8:30 AM-5:00 PM and by appointment.


Dr. Johnson is the Charles E. Putman University Professor of Radiology, Professor of Physics, and Biomedical Engineering, and Director of the Duke Center for In Vivo Microscopy (CIVM). The CIVM is an NIH/NIBIB national Biomedical Technology Resource Center with a mission to develop novel technologies for preclinical imaging (basic sciences) and apply the technologies to critical biomedical questions. Dr. Johnson was one of the first researchers to bring Paul Lauterbur's vision of magnetic resonance (MR) microscopy to practice as described in his paper, "Nuclear magnetic resonance imaging at microscopic resolution" (J Magn Reson 68:129-137, 1986). Dr. Johnson is involved in both the engineering physics required to extend the resolution of MR imaging and in a broad range of applications in the basic sciences.

Education & Training

  • Ph.D., Duke University 1974

Selected Grants

The Duke Preclinical Research Resources for Quantitative Imaging Biomarkers awarded by National Institutes of Health (PD/PI). 2017 to 2022

Training in Medical Imaging awarded by National Institutes of Health (Mentor). 2003 to 2019

Quantitative MR Microscopy of Phenotypic Biomarkers in Alzheimer's Disease awarded by National Institutes of Health (Mentor). 2013 to 2018

Integrated Center For In Vivo Microscopy awarded by National Institutes of Health (Principal Investigator). 2003 to 2018

Waxholm Space for Rodent Neuroinformatics awarded by University of Pennsylvania (Principal Investigator). 2016 to 2018

Serial Block Face Scanning Electron Microscope awarded by National Institutes of Health (Major User). 2016 to 2018

Ultra-resolution imaging of brain circuitry and its development in mental health awarded by National Institutes of Health (Co Investigator). 2013 to 2016

MRI Corticography (MRCoG): Micro-scale Human Cortical Imaging awarded by University of California - Berkeley (Collaborator). 2014 to 2016

Prevention of Temporal Lobe Epilepsy awarded by National Institutes of Health (Co Investigator). 2012 to 2015

Whole brain multimodal microscopy of an apoptosis reporter mouse awarded by National Institutes of Health (Collaborator). 2012 to 2014


Paxinos, G, Watson, C, Calabrese, E, Badea, A, and Johnson, G. An MRI/DTI Atlas of the Rat Brain. Elsevier, Academic Press, May 28, 2015.

Spiliopoulos, D, Kagadis, GC, Karnabatidis, D, Johnson, GA, and Badea, CT. "Digital Subtracted Angiography of Small Animals (Accepted)." Handbook of Small Animal Imaging: Preclinical Imaging, Therapy, and Applications. Ed. GC Kagadis, NL Ford, GK Loudos, and D Karnabatidis. Taylor & Francis Books, Inc., CRC Press, March 3, 2016. 67-75. (Chapter)

Wang, N, Anderson, RJ, Badea, A, Cofer, G, Dibb, R, Qi, Y, and Johnson, GA. "Whole mouse brain structural connectomics using magnetic resonance histology." Brain Structure & Function (September 17, 2018). Full Text

Nouls, JC, Badea, A, Anderson, RBJ, Cofer, GP, and Allan Johnson, G. "Diffusion tensor imaging using multiple coils for mouse brain connectomics." Nmr in Biomedicine 31.6 (June 2018): e3921-null. Full Text

Wei, H, Gibbs, E, Zhao, P, Wang, N, Cofer, GP, Zhang, Y, Johnson, GA, and Liu, C. "Susceptibility tensor imaging and tractography of collagen fibrils in the articular cartilage." Magnetic resonance in medicine 78.5 (November 2017): 1683-1690. Full Text

Vetreno, RP, Yaxley, R, Paniagua, B, Johnson, GA, and Crews, FT. "Adult rat cortical thickness changes across age and following adolescent intermittent ethanol treatment." Addiction Biology 22.3 (May 2017): 712-723. Full Text

Xie, L, Bennett, KM, Liu, C, Johnson, GA, Zhang, JL, and Lee, VS. "MRI tools for assessment of microstructure and nephron function of the kidney." American Journal of Physiology Renal Physiology 311.6 (December 2016): F1109-F1124. (Review) Full Text Open Access Copy

Wei, H, Xie, L, Dibb, R, Li, W, Decker, K, Zhang, Y, Johnson, GA, and Liu, C. "Imaging whole-brain cytoarchitecture of mouse with MRI-based quantitative susceptibility mapping." Neuroimage 137 (August 2016): 107-115. Full Text

Xie, L, Layton, AT, Wang, N, Larson, PEZ, Zhang, JL, Lee, VS, Liu, C, and Johnson, GA. "Dynamic contrast-enhanced quantitative susceptibility mapping with ultrashort echo time MRI for evaluating renal function." American Journal of Physiology Renal Physiology 310.2 (January 2016): F174-F182. Full Text

Subashi, E, Cordero, FJ, Halvorson, KG, Qi, Y, Nouls, JC, Becher, OJ, and Johnson, GA. "Tumor location, but not H3.3K27M, significantly influences the blood-brain-barrier permeability in a genetic mouse model of pediatric high-grade glioma." Journal of neuro-oncology 126.2 (January 2016): 243-251. Full Text

Calabrese, E, Badea, A, Cofer, G, Qi, Y, and Johnson, GA. "A Diffusion MRI Tractography Connectome of the Mouse Brain and Comparison with Neuronal Tracer Data." Cerebral Cortex (New York, N.Y. : 1991) 25.11 (November 2015): 4628-4637. Full Text Open Access Copy

Calabrese, E, Badea, A, Coe, CL, Lubach, GR, Shi, Y, Styner, MA, and Johnson, GA. "A diffusion tensor MRI atlas of the postmortem rhesus macaque brain." Neuroimage 117 (August 2015): 408-416. Full Text


Johnson, GA, Anderson, RJ, Cook, JJ, Long, C, and Badea, A. "Image-processing pipelines: Applications in magnetic resonance histology." January 1, 2016. Full Text

Calabrese, E, Hickey, P, Hulette, C, Zhang, J, Parente, B, Lad, SP, and Johnson, GA. "Postmortem Diffusion MRI of the Human Brainstem and Thalamus for Deep Brain Stimulator Electrode Localization." September 2015.

Johnson, GA, Badea, A, Calabrese, E, Liu, C, and Xie, L. "Magnetic resonance histology—Applications in toxicology." September 2014. Full Text

Clark, DP, Johnson, GA, and Badea, CT. "Robust material decomposition for spectral CT." January 1, 2014. Full Text

Badea, CT, Befera, N, Clark, D, Qi, Y, and Johnson, GA. "Dual-energy micro-CT imaging of pulmonary airway obstruction: Correlation with micro-SPECT." January 1, 2014. Full Text

Johnston, SM, Johnson, GA, and Badea, CT. "Helical dual source cone-beam micro-CT." January 1, 2014.

Clark, D, Johnston, SM, Johnson, GA, and Badea, CT. "The effect of scatter correction on dual energy micro-CT." 2013. Full Text